SORCS1 Polyklonaler Antikörper

SORCS1 Polyklonal Antikörper für FC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18839-1-AP

Synonyme

hSorCS, SORCS1

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Galerie der Validierungsdaten

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  WB analysis of Y79 using 18839-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18839-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 18839-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 18839-1-AP

  1X10^6 HeLa cells were stained with 0.2ug SORCS1 antibody (18839-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, HEK-293-Zellen, Maushirngewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18839-1-AP bindet in WB, FC,ELISA SORCS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: sortilin-related VPS10 domain containing receptor 1
• Berechnetes Molekulargewicht: 1168 aa, 130 kDa
• Beobachtetes Molekulargewicht: 130 kDa, 60-80 kDa
• GenBank-Zugangsnummer: BC131597
• Gene symbol: SORCS1
• Gene ID (NCBI): 114815
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SorCS1 is a 130 kDa type I transmembrane protein, belonging to Vps10p-domain (Vps10p-D) receptor family that comprises an N-terminal Vps10p-D, a leucine-rich domain, a single transmembrane domain, and a short cytoplasmic domain. SorCS1 is synthesized as a proprotein, which is converted to the mature receptor in late Golgi compartments by cleavage of the N-terminal propeptide. Alternative splicing of human SorCS1 results in four isoforms with different cytoplasmic domains and differential expression in tissues. This antibody was generated against the C-terminal region of human SorCS1 and recognizes various forms of SorCS1. Multiple bands around 100-130 kDa as well as 60-80 kDa have been detected in Y79 cells by western blot assay.