SORCS1 Polyklonaler Antikörper

SORCS1 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 23002-1-AP

Synonyme

hSorCS, SORCS, SORCS1

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Galerie der Validierungsdaten

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  WB analysis using 23002-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23002-1-AP (SORCS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 23002-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23002-1-AP (SORCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 23002-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 23002-1-AP (SORCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 23002-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23002-1-AP (SORCS1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 23002-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23002-1-AP (SORCS1 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HeLa using 23002-1-AP

  1X10^6 HeLa cells were stained with 0.2ug SORCS1 antibody (23002-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Hirngewebe, Mausnierengewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

23002-1-AP bindet in WB, IHC, FC, ELISA SORCS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SORCS1 fusion protein Ag19199
• Vollständiger Name: sortilin-related VPS10 domain containing receptor 1
• Berechnetes Molekulargewicht: 1168 aa, 130 kDa
• Beobachtetes Molekulargewicht: 60-80 kDa
• GenBank-Zugangsnummer: BC131597
• Gene symbol: SORCS1
• Gene ID (NCBI): 114815
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SORCS1, also named as SorCS, belongs to the family of VPS10 domain-containing receptors. SorCS1 immunoreactivity is widespread in a population of neurons throughout the brain. Two different types of cellular localization were observed. Most SorCS1 immunoreactive neurons exhibit a punctate cytoplasmic staining which extends into the dendrites, whilst occassionally SorCS1 neuronal immunoreactivity is associated with the plasma membrane.

Publikationen

Species	Application	Title
human	WB	Aging (Albany NY); CircSPIDR acts as a tumour suppressor in cervical adenocarcinoma by sponging miR-431-5p and regulating SORCS1 and CUBN expression.; Authors - Junfen Xu