Sorbitol dehydrogenase Polyklonaler Antikörper

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Sorbitol dehydrogenase Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 15881-1-AP

Synonyme

SORD, (R,R)-butanediol dehydrogenase, EC:1.1.1.-, EC:1.1.1.14, EC:1.1.1.4

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  WB analysis of HeLa using 15881-1-AP

  WB result of Sorbitol dehydrogenase antibody (15881-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Sorbitol dehydrogenase transfected HeLa cells.

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  WB analysis using 15881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15881-1-AP (Sorbitol dehydrogenase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15881-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15881-1-AP (Sorbitol dehydrogenase antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15881-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15881-1-AP (Sorbitol dehydrogenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15881-1-AP

  IP result of anti-Sorbitol dehydrogenase (IP:15881-1-AP, 4ug; Detection:15881-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human ovary cancer using 15881-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15881-1-AP (Sorbitol dehydrogenase antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Nierengewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 1 publications below

Produktinformation

15881-1-AP bindet in WB, IHC, IF, IP, ELISA Sorbitol dehydrogenase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Sorbitol dehydrogenase fusion protein Ag8651
• Vollständiger Name: sorbitol dehydrogenase
• Berechnetes Molekulargewicht: 357 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC021085
• Gene symbol: SORD
• Gene ID (NCBI): 6652
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SORD(Sorbitol dehydrogenase) is also named as L-iditol 2-dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. It catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. This protein can form a homotetramer(PMID:12962626). Measurement of SORD is a specific indicator of liver cell damage and parenchymal.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Endogenous fructose production and metabolism in the liver contributes to the development of metabolic syndrome.; Authors - Lanaspa Miguel A MA
mouse	WB,IF	Reproduction; Nicotine reduces Sord expression and tyrosine phosphorylation in capacitated sperm.; Authors - Jingbo Dai
mouse	WB	J Mol Med (Berl); Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.; Authors - Yu Sun
rat	WB	Chem Biol Interact; Hepatocyte ferroptosis contributes to anti-tuberculosis drug-induced liver injury: Involvement of the HIF-1α/SLC7A11/GPx4 axis; Authors - Yining Liu
mouse	WB	Nutrients; Endogenous Fructose Production and Metabolism Drive Metabolic Dysregulation and Liver Disease in Mice with Hereditary Fructose Intolerance; Authors - Ana Andres-Hernando
mouse	WB	J Hepatol; Ketohexokinase-C regulates global protein acetylation to decrease carnitine palmitoyltransferase 1a-mediated fatty acid oxidation; Authors - Robert N Helsley