SOX15 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SOX15 Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 25415-1-AP

Synonyme

Protein SOX 12, Protein SOX 15, Protein SOX 20, SOX12, SOX15, SOX20, SOX26, SOX27

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  IHC staining of mouse embryo using 25415-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 25415-1-AP (SOX15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 25415-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 25415-1-AP (SOX15 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse embryo using 25415-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 25415-1-AP (SOX15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausembryogewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausembryogewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

25415-1-AP bindet in WB, IHC, IF-P, ELISA SOX15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOX15 fusion protein Ag22032
• Vollständiger Name: SRY (sex determining region Y)-box 15
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC000985
• Gene symbol: SOX15
• Gene ID (NCBI): 6665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SOX15, also named as SOX12, SOX20, is a 223 amino acid protein, which contains one HMG box DNA-binding domain. SOX15 is widely expressed in fetal and adult tissues and highest level is found in fetal spinal cord and adult brain and testis. SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/β-catenin signaling. Sox15 is required for skeletal muscle regeneration and is up regulated in the embryonic mouse testis.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Transcription factor SOX15 regulates stem cell pluripotency and promotes neural fate during differentiation by activating the neurogenic gene Hes5; Authors - Eun-Bee Choi ; KO Validated
human,mouse	WB	Oncogene; CircVPS8 promotes the malignant phenotype and inhibits ferroptosis of glioma stem cells by acting as a scaffold for MKRN1, SOX15 and HNF4A; Authors - Jinpeng Hu
mouse	IF	MicroPubl Biol; A commonly used anti-SOX15 antibody fails to demonstrate specificity in mouse embryos; Authors - Farina Aziz