SP6 Polyklonaler Antikörper

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• KD/KO Validated

SP6 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 21234-1-AP

Synonyme

AI1K, EPFN, EPIPROFIN, KLF14, Krueppel-like factor 14

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  WB analysis of fetal human brain using 21234-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21234-1-AP (KLF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21234-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21234-1-AP (KLF14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 21234-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 21234-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 21234-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 21234-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of Caco-2 using 21234-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SP6 antibody (21234-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

21234-1-AP bindet in WB, IHC, IF/ICC, ChIP, ELISA SP6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SP6 fusion protein Ag15707
• Vollständiger Name: Sp6 transcription factor
• Berechnetes Molekulargewicht: 376 aa, 40 kDa
• GenBank-Zugangsnummer: BC103951
• Gene symbol: SP6
• Gene ID (NCBI): 80320
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SP6 (chromosome 17) is a 376 amino acid protein, which localizes in nucleus. SP6 is a transcription factor of the SP/KLF family and an indispensable regulator of the morphological dynamics of ameloblast differentiation during tooth development. The levels of sp6 protein expressed in various tissues in the embryo. This antibody detects the SP6 protein. It can't detect the KLF14/BTEB5.

This antibody was previously listed as a KLF14 antibody, because the SP6 gene on chromosome 17q21.32 has been referred to as KLF14 in the literature. SP6 should not be confused with the KLF14 gene on chromosome 7q32.2.

Publikationen

Species	Application	Title
human	WB,IHC	Int J Biol Sci; The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer.; Authors - Guiyang Wu
rat,mouse,human	WB,IHC,IF,ChIP	FEBS J; Molecular mechanism for transcriptional regulation of the parathyroid hormone gene by Epiprofin; Authors - Takashi Nakamura