SPAG11A Polyklonaler Antikörper

SPAG11A Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 16770-1-AP

Synonyme

EP2, HE2, Protein EP2, SPAG11A, Sperm antigen HE2, sperm associated antigen 11A

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Galerie der Validierungsdaten

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  WB analysis of human testis using 16770-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16770-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

16770-1-AP bindet in WB, IHC,ELISA SPAG11A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPAG11A fusion protein Ag9846
• Vollständiger Name: sperm associated antigen 11A
• Berechnetes Molekulargewicht: 123 aa, 14 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC058833
• Gene symbol: SPAG11A
• Gene ID (NCBI): 653423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sperm binding proteins and their C-terminal peptides of the Sperm AssociatedAntigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671).SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing.This antibody is specific to SPAG11.

Publikationen

Species	Application	Title
mouse	WB,IHC	Reprod Biol Endocrinol; Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner.; Authors - Pujianto Dwi A DA