SPAG16 Polyklonaler Antikörper

SPAG16 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16883-1-AP

Synonyme

Sperm-associated antigen 16 protein, Pf20 protein homolog, PF20

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  WB analysis of rat testis using 16883-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16883-1-AP (SPAG16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16883-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16883-1-AP (SPAG16 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 16883-1-AP

  IP result of anti-SPAG16 (IP:16883-1-AP, 4ug; Detection:16883-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of human brain using 16883-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16883-1-AP (SPAG16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 16883-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16883-1-AP (SPAG16 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 16883-1-AP

  Immunofluorescent analysis of HepG2 cells, using SPAG16 antibody 16883-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 16883-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPAG16 antibody (16883-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhodengewebe, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

16883-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SPAG16 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPAG16 fusion protein Ag10348
• Vollständiger Name: sperm associated antigen 16
• Berechnetes Molekulargewicht: 631 aa, 71 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC009614
• Gene symbol: SPAG16
• Gene ID (NCBI): 79582
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAG16 (also known as PF20) is an orthologue of Chlamydomonas PF20, a component of the alga axoneme central apparatus that is required for flagellar motility. SPAG16 is highly expressed in the testis, low abundant or absent from other tissues. The SPAG16 gene encodes two major proteins, a 71-kDa SPAG16L and a smaller 35-kDa SPAG16S nuclear protein that represents the C-terminus of SPAG16L. SPAG16L was detected abundantly in the cytoplasm, while SPAG16S was detected in both cytoplasm and nucleus. This antibody was raised against the N-terminal region of SPAG16 and mainly recognizes SPAG16L.

Publikationen

Species	Application	Title
human	WB	Ecotoxicol Environ Saf; Microcystin leucine arginine induces human sperm damage: Involvement of the Ca2+/CaMKKβ/AMPK pathway; Authors - Xing Guo
mouse	IF	Cell Mol Life Sci; CFAP65 is essential for C2a projection integrity in axonemes: implications for organ-specific ciliary dysfunction and infertility; Authors - Jinyi Chen