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  • Featured Product
  • KD/KO Validated

SPAG5 Polyklonaler Antikörper

SPAG5 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-14726

Synonyme

Astrin, DEEPEST, hMAP126, MAP126, SPAG5, sperm associated antigen 5

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated SPAG5 Polyclonal antibody (CL594-14726)

    IF Staining of HeLa using CL594-14726

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 SPAG5 antibody (CL594-14726) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated SPAG5 Polyclonal antibody (CL594-14726)

    FC experiment of HeLa using CL594-14726

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SPAG5 (CL594-14726) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-14726 bindet in IF, FC (Intra) SPAG5 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SPAG5 fusion protein Ag6485
Vollständiger Name sperm associated antigen 5
Berechnetes Molekulargewicht 134 kDa
Beobachtetes Molekulargewicht 135 kDa, 150 kDa
GenBank-ZugangsnummerBC000322
Gene symbol SPAG5
Gene ID (NCBI) 10615
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 SPAG5 antibody CL594-14726Protokoll herunterladen
FC protocol for CL594 SPAG5 antibody CL594-14726Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen