SPAG7 Polyklonaler Antikörper

SPAG7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16294-1-AP

Synonyme

ACRP, FSA 1, SPAG7, sperm associated antigen 7

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  WB analysis of mouse ovary using 16294-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16294-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16294-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16294-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 16294-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 16294-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Eierstockgewebe, Maushirngewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16294-1-AP bindet in WB, IHC, ELISA SPAG7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPAG7 fusion protein Ag9471
• Vollständiger Name: sperm associated antigen 7
• Berechnetes Molekulargewicht: 227 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-31 kDa
• GenBank-Zugangsnummer: BC011934
• Gene symbol: SPAG7
• Gene ID (NCBI): 9552
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAG7 encodes the sperm-associated antigen 7, which is widely expressed. The SPAG7 protein contains two nuclear localisation signals and a R3H domain. The domain is predicted to bind ssDNA or ssRNA in a sequence-specific manner. This might indicate a potential role of the protein in response to viruses or free DNAs or RNAs.