SPANXE Polyklonaler Antikörper

SPANXE Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15870-1-AP

Synonyme

SPANX C, SPANX A2, Nuclear-associated protein SPAN-Xc, CT11.3, Cancer/testis-associated protein CTp11

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  WB analysis of A375 using 15870-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15870-1-AP (SPANXE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 15870-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 15870-1-AP (SPANXE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 15870-1-AP

  Immunofluorescent analysis of A375 cells using 15870-1-AP (SPANXE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, Maushautgewebe
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15870-1-AP bindet in WB, IF/ICC, ELISA SPANXE und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPANXE fusion protein Ag8572
• Vollständiger Name: SPANX family, member E
• Berechnetes Molekulargewicht: 97 aa, 11 kDa
• Beobachtetes Molekulargewicht: 15-20 kDa
• GenBank-Zugangsnummer: BC005382
• Gene symbol: SPANXE
• Gene ID (NCBI): 171489
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPANXE (Sperm protein associated with the nucleus on the X chromosome E), also known as SPANX A2 or SPANX C, is a member of the SPANX family. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene encodes a sperm protein that contains a consensus nuclear localization signal but, although a role in spermatogenesis is suggested, the specific function of this family member has not yet been determined.