SPARC Monoklonaler Antikörper

SPARC Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

1A2C1

Kat-Nr. : 66426-1-Ig

Synonyme

Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA375-Zellen, humanes Hodengewebe
Erfolgreiche Detektion in IHChumanes Plazenta-Gewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCC6-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66426-1-Ig bindet in WB, IHC, IF/ICC, ELISA SPARC und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen SPARC fusion protein Ag7390
Vollständiger Name secreted protein, acidic, cysteine-rich (osteonectin)
Berechnetes Molekulargewicht 35 kDa
Beobachtetes Molekulargewicht 38 kDa, 52 kDa
GenBank-ZugangsnummerBC004974
Gene symbol SPARC
Gene ID (NCBI) 6678
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SPARC antibody 66426-1-IgProtokoll herunterladen
IHC protocol for SPARC antibody 66426-1-IgProtokoll herunterladenl
IF protocol for SPARC antibody 66426-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,mouseIHC

Adv Sci (Weinh)

Elevated SPARC Disrupts the Intestinal Barrier Integrity in Crohn's Disease by Interacting with OTUD4 and Activating the MYD88/NF-κB Pathway

Authors - Jiayu Wang
human,mouseWB

Int J Cancer

Tissue-resident CXCR4+ macrophage as a poor prognosis signature promotes pancreatic ductal adenocarcinoma progression

Authors - Zhenyu Liao
humanWB

Br J Cancer

Inactivation of 3-hydroxybutyrate dehydrogenase type 2 promotes proliferation and metastasis of nasopharyngeal carcinoma by iron retention.

Authors - Bo Li
humanWB

Front Endocrinol (Lausanne)

Secreted Protein Acidic and Rich in Cysteine Mediates the Development and Progression of Diabetic Retinopathy.

Authors - Liying Luo
mouseWB

Exp Cell Res

Sorafenib derivatives-functionalized gold nanoparticles confer protection against tumor angiogenesis and proliferation via suppression of EGFR and VEGFR-2.

Authors - Wei Huang
humanWB

Pathol Res Pract

Downregulation of NEBL promotes migration and invasion of clear cell renal cell carcinoma by inducing epithelial-mesenchymal transition

Authors - Ran Zhao