SPATS2L Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SPATS2L Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 16938-1-AP

Synonyme

LOC26010, SPATS2 like protein, SP1224, SGNP, DNAPTP6

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  WB analysis of HepG2 using 16938-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16938-1-AP (SPATS2L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16938-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16938-1-AP (SPATS2L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 16938-1-AP

  Immunofluorescent analysis of HepG2 cells, using LOC26010 antibody 16938-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of Saos-2 using 16938-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using SPATS2L antibody (16938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Saos-2 cells, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16938-1-AP bindet in WB, IHC, IF/ICC, ELISA SPATS2L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPATS2L fusion protein Ag10438
• Vollständiger Name: viral DNA polymerase-transactivated protein 6
• Berechnetes Molekulargewicht: 488 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54-62 kDa
• GenBank-Zugangsnummer: BC018736
• Gene symbol: SPATS2L
• Gene ID (NCBI): 26010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPATS2L encodes spermatogenesis associated and serine-rich 2-like protein, also named DNAPTP6 (DNA polymerase transactivated protein 6) in human (PMID:11230166). SPATS2L acts as an important regulator of β2-adrenergic receptor down-regulation (PubMed: 25562107). MiR-1269a down-regulates the expression of SPATS2L and LRP6, thereby inhibiting the proliferation of liver cancer cells (PMID: 28081866, PMID:35252180). SPATS2L has several isoforms by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu
human	IHC,WB	Sci Rep; Characteristic DNA methylation profiles of chorionic villi in recurrent miscarriage.; Authors - Yosuke Matsumoto ; KD Validated
human	WB	J Transl Med; Spermatogenesis associated serine rich 2 like plays a prognostic factor and therapeutic target in acute myeloid leukemia by regulating the JAK2/STAT3/STAT5 axis; Authors - Fenglin Li ; KD Validated
mouse	IF	Sci Adv; RNA G-quadruplex organizes stress granule assembly through DNAPTP6 in neurons; Authors - Sefan Asamitsu