SPCS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SPCS1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 11847-1-AP

Synonyme

SPC12, SPC1, SPase 12 kDa subunit, HSPC033

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  WB analysis using 11847-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11847-1-AP (SPCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11847-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11847-1-AP (SPCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skin using 11847-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11847-1-AP (SPCS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IF Staining of BxPC-3 using 11847-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using SPCS1 antibody (11847-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hautgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below

Produktinformation

11847-1-AP bindet in WB, IF/ICC, ELISA SPCS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPCS1 fusion protein Ag2407
• Vollständiger Name: signal peptidase complex subunit 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 102 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC000884
• Gene symbol: SPCS1
• Gene ID (NCBI): 28972
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nature; A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.; Authors - Rong Zhang ; KO Validated
human	WB	PLoS Pathog; Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2.; Authors - Suzuki Ryosuke R ; KD Validated
	WB	PLoS Pathog; SPCS1-Dependent E2-p7 processing determines HCV Assembly efficiency.; Authors - Nabeel Alzahrani
human	WB	Mol Cell; Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signaling pathway; Authors - Iqbal Dulloo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joao (Verified Customer) (10-09-2019)

Specific, single band (~12kDa), low background

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: 293T

SPCS1 Antibody Western Blot, validation (1:500 dilution) in 293T (Cat no:11847-1-AP)