SPDEF Polyklonaler Antikörper

SPDEF Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 11467-1-AP

Synonyme

PDEF, Prostate derived Ets factor, Prostate specific Ets, PSE, RP11 375E1__A.3, SPDEF

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 11467-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11467-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11467-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11467-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11467-1-AP bindet in WB, IHC, IF, ELISA SPDEF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPDEF fusion protein Ag2020
• Vollständiger Name: SAM pointed domain containing ets transcription factor
• Berechnetes Molekulargewicht: 335 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38-44 kDa
• GenBank-Zugangsnummer: BC021299
• Gene symbol: SPDEF
• Gene ID (NCBI): 25803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell; A modular master regulator landscape controls cancer transcriptional identity.; Authors - Evan O Paull
human	WB,IHC	Clin Sci (Lond); Gut microbiota-derived butyrate regulates gut mucus barrier repair by activating the macrophage/WNT/ERK signaling pathway.; Authors - Liping Liang
human	WB,IF	Phytomedicine; Resveratrol inhibits MUC5AC expression by regulating SPDEF in lung cancer cells.; Authors - Yu-Hua Lin
mouse	WB	Food Funct; EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.; Authors - Jian Fang
mouse	WB	Immunobiology; Lactobacillus rhamnosus 76 alleviates airway inflammation in ovalbumin-allergic mice and improves mucus secretion by down-regulating STAT6/SPDEF pathway; Authors - Yangfan Hou
rat	WB	Dig Dis Sci; Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats.; Authors - Linhao Zhang