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SPI1 Polyklonaler Antikörper
SPI1 Polyklonal Antikörper für WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 55100-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IF | See 1 publications below |
Produktinformation
55100-1-AP bindet in WB, IF, ELISA SPI1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | spleen focus forming virus (SFFV) proviral integration oncogene spi1 |
| Berechnetes Molekulargewicht | 31 kDa |
| Beobachtetes Molekulargewicht | 31-42 kDa |
| GenBank-Zugangsnummer | NM_003120 |
| Gene symbol | SPI1 |
| Gene ID (NCBI) | 6688 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
SPI1 binds to the PU-box which is a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. SPI1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. It also binds RNA and may modulate pre-mRNA splicing. The antibody is specific to SPI1. SPI1 can be detected 37-42 kDa (PMID: 28362429, 31586032, 28586009).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for SPI1 antibody 55100-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Mucosal Immunol IL-33/ST2/IL-9/IL-9R signaling disrupts ocular surface barrier in allergic inflammation. | ||
Sci Rep Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine. | ||
J Leukoc Biol miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression.
| ||
Exp Cell Res An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1 | ||
Cell Rep A CLIC1 network coordinates matrix stiffness and the Warburg effect to promote tumor growth in pancreatic cancer |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Andrew (Verified Customer) (08-09-2021) | When used in our capillary electrophoresis equipment (JESS), the proteintech antibody for SPI1 seemed to show specific binding to a protein at around 60KD. We believe this may be recognising a dimerized form of the protein. This detection was consistent between lanes and responded as expected to varying amounts of sample protein and antibody concentration.
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