SPR Polyklonaler Antikörper

SPR Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 16822-1-AP

Synonyme

EC:1.1.1.153, SDR38C1, Sepiapterin reductase

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  WB analysis using 16822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 16822-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 16822-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16822-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16822-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16822-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16822-1-AP

  IP result of anti-SPR (IP:16822-1-AP, 4ug; Detection:16822-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver cancer using 16822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16822-1-AP (SPR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16822-1-AP (SPR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16822-1-AP

  Immunofluorescent analysis of Hela cells, using SPR antibody 16822-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Lebergewebe, K-562-Zellen, Rattenherzgewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

16822-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SPR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPR fusion protein Ag10395
• Vollständiger Name: sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase)
• Berechnetes Molekulargewicht: 261 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC017310
• Gene symbol: SPR
• Gene ID (NCBI): 6697
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Chem Biol; Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers.; Authors - Mariluz Soula
mouse	WB	Acta Physiol (Oxf); Tetrahydrobiopterin synthesis and metabolism is impaired in dystrophin-deficient mdx mice and humans.; Authors - Angus Lindsay
	WB	Cell Death Dis; Identification of genes supporting cold resistance of mammalian cells: lessons from a hibernator; Authors - Masamitsu Sone
human	WB	Phytomedicine; Cimifugin suppresses type 2 airway inflammation by binding to SPR and regulating its protein expression in a non-enzymatic manner; Authors - Xiaoqun Gu