SPTLC2 Polyklonaler Antikörper

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• KD/KO Validated

SPTLC2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 51012-2-AP

Synonyme

KIAA0526, LCB 2, LCB2, LCB2a, Serine palmitoyltransferase 2, SPT 2, SPT2, SPTLC2

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Galerie der Validierungsdaten

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  WB analysis using 51012-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51012-2-AP (SPTLC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 51012-2-AP

  WB result of SPTLC2 antibody (51012-2-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC2 transfected HEK-293 cells.

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  WB analysis of mouse brain using 51012-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51012-2-AP (SPTLC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 51012-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 51012-2-AP (SPTLC2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 51012-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 51012-2-AP (SPTLC2 antibody) at dilution of 1:200 (under 0x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HepG2-Zellen, HT-29-Zellen, Maushirngewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

51012-2-AP bindet in WB, IHC, IF, FC, ELISA SPTLC2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: serine palmitoyltransferase, long chain base subunit 2
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC005123
• Gene symbol: SPTLC2
• Gene ID (NCBI): 9517
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Gastroenterology; IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis.; Authors - Debanjali Dasgupta
human	WB,FC	Nat Microbiol; Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection.; Authors - Robert C Orchard
mouse	WB,IF	Mol Cell Proteomics; Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.; Authors - Campbell-Valois François-Xavier FX
mouse	WB	Cell Death Dis; Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function.; Authors - Zhiqiang Li ; KO Validated
mouse	WB	J Biol Chem; Liver-specific deficiency of serine palmitoyltransferase subunit 2 decreases plasma sphingomyelin and increases apolipoprotein E levels.; Authors - Li Zhiqiang Z
mouse	WB	J Biol Chem; Macrophage-specific de novo synthesis of ceramide is dispensable for inflammasome-driven inflammation and insulin-resistance in obesity.; Authors - Christina D Camell