SRA1 Polyklonaler Antikörper

SRA1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 24655-1-AP

Synonyme

PP7684, SRAP, Steroid receptor RNA activator 1, Steroid receptor RNA activator protein

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  WB analysis of A2780 using 24655-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24655-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 24655-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24655-1-AP (SRA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24655-1-AP (SRA1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24655-1-AP (SRA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24655-1-AP (SRA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 24655-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SRA1 antibody (24655-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of MCF-7 using 24655-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug SRA1 Polyclonal antibody (24655-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 1 publications below

Produktinformation

24655-1-AP bindet in WB, IHC, IF/ICC, ELISA SRA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRA1 fusion protein Ag20082
• Vollständiger Name: steroid receptor RNA activator 1
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 30 kDa, 33 kDa
• GenBank-Zugangsnummer: BC067895
• Gene symbol: SRA1
• Gene ID (NCBI): 10011
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription. This polyclonal antibody recognizes endogenous SRA1 isoforms which migrate as a doublet on SDS-PAGE gels (PMID: 12565891; 23907597).

Publikationen

Species	Application	Title
		Adv Sci (Weinh); Pyruvate Carboxylase in Macrophages Aggravates Atherosclerosis by Regulating Metabolism Reprogramming to Promote Inflammatory Responses Through the Hypoxia-Inducible Factor-1 Signaling Pathway; Authors - Ling-Na Zhao
mouse	WB	Free Radic Biol Med; Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages; Authors - Wenchao Li,
mouse,human	WB	Phytomedicine; Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux; Authors - Jianfei Mu
mouse,human	WB	Arterioscler Thromb Vasc Biol; RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction.; Authors - Yan-Wei Hu
human	WB	J Lipid Res; A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent Signal Transduction Pathway Is Essential for the Regulation of Cholesterol Homeostasis and Inflammatory Reactions.; Authors - Yan-Wei Hu
human	WB	J Lipid Res; VNN1 Promotes Atherosclerosis Progression in apoE Deficiency Mice Fed a High Fat High Cholesterol Diet.; Authors - Yan-Wei Hu