SRD5A1 Monoklonaler Antikörper

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• KD/KO Validated

SRD5A1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1D5D8

Kat-Nr. : 66329-1-Ig

Synonyme

S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1

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  WB analysis of human testis using 66329-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 66329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 66329-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66329-1-Ig(SRD5A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HEPG2 using 66329-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HEPG2 cells using SRD5A1 antibody (66329-1-Ig, Clone: 1D5D8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

66329-1-Ig bindet in WB, IHC, IF/ICC, ELISA SRD5A1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SRD5A1 fusion protein Ag23351
• Vollständiger Name: steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC007033
• Gene symbol: SRD5A1
• Gene ID (NCBI): 6715
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Steroid 5-alpha-reductase (SRD5A1)   is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.

Publikationen

Species	Application	Title
human	WB	Cancer Sci; Modulation of AKR1C2 by curcumin decreases testosterone production in prostate cancer.; Authors - Hisamitsu Ide
	WB	Sci Rep; Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells.; Authors - Mitsuki Nozaki
human	WB,IHC	Front Oncol; Steroid 5α-Reductase Type I Induces Cell Viability and Migration via Nuclear Factor-κB/Vascular Endothelial Growth Factor Signaling Pathway in Colorectal Cancer.; Authors - Rongfang Wei ; KD Validated
human	WB	In Vivo; Effects of Curcumin Combined With the 5-alpha Reductase Inhibitor Dutasteride on LNCaP Prostate Cancer Cells.; Authors - Akinori Nakayama
human	WB	Cell Death Dis; Suppression of steroid 5α-reductase type I promotes cellular apoptosis and autophagy via PI3K/Akt/mTOR pathway in multiple myeloma.; Authors - Renjie Dou ; KD Validated
mouse	IHC	Nat Commun; The estrous cycle modulates early-life adversity effects on mouse avoidance behavior through progesterone signaling; Authors - Blake J Laham