• Featured Product
  • KD/KO Validated

SREBF1 Polyklonaler Antikörper

SREBF1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(285)

Kat-Nr. : 14088-1-AP

Synonyme

SREBP 1c, SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, L02-Zellen, MCF-7-Zellen, Mauslebergewebe, Rattenlebergewebe
Erfolgreiche IPL02-Zellen
Erfolgreiche Detektion in IHChumanes Nierengewebe, humanes Skelettmuskelgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14088-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA SREBF1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Hausschwein, Huhn, Maus, Ratte, Rind, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SREBF1 fusion protein Ag5219
Vollständiger Name sterol regulatory element binding transcription factor 1
Berechnetes Molekulargewicht 1177 aa, 125 kDa
Beobachtetes Molekulargewicht 125 kDa, 68 kDa
GenBank-ZugangsnummerBC063281
Gene symbol SREBF1
Gene ID (NCBI) 6720
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125 kDa precursor form and the 68 kDa mature form of human SREBF1.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SREBF1 antibody 14088-1-APProtokoll herunterladen
IHC protocol for SREBF1 antibody 14088-1-APProtokoll herunterladenl
IF protocol for SREBF1 antibody 14088-1-APProtokoll herunterladen
IP protocol for SREBF1 antibody 14088-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cancer Cell

KMT2C deficiency drives transdifferentiation of double-negative prostate cancer and confer resistance to AR-targeted therapy

Authors - Jiacheng Guo
humanWB

Nature

A metastasis map of human cancer cell lines.

Authors - Xin Jin
  • KO Validated
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
humanWB

Cell Metab

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Authors - Qidong Li
  • KD Validated
mouseIHC

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
mouseWB

Nat Commun

Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity

Authors - Jinjie Duan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Isha (Verified Customer) (05-18-2022)

Antibody worked perfect at 1:1000 dilution for W.B. and 1:500 for IF. And for chIP 3ug/ml worked best.

  • Applications: Western Blot, Immunofluorescence, ChIP