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SREBF1 Polyklonaler Antikörper
SREBF1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (5)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14088-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, L02-Zellen, MCF-7-Zellen, Mauslebergewebe, Rattenlebergewebe | 
| Erfolgreiche IP | L02-Zellen | 
| Erfolgreiche Detektion in IHC | humanes Nierengewebe,  humanes Skelettmuskelgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:50-1:500 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 10 publications below | 
| WB | See 256 publications below | 
| IHC | See 34 publications below | 
| IF | See 29 publications below | 
| IP | See 2 publications below | 
| CoIP | See 2 publications below | 
| ChIP | See 6 publications below | 
Produktinformation
14088-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA SREBF1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte | 
| In Publikationen genannte Reaktivität | human, Affe, Hausschwein, Huhn, Maus, Ratte, Rind, Ziege | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | SREBF1 fusion protein Ag5219 | 
| Vollständiger Name | sterol regulatory element binding transcription factor 1 | 
| Berechnetes Molekulargewicht | 1177 aa, 125 kDa | 
| Beobachtetes Molekulargewicht | 125 kDa, 68 kDa | 
| GenBank-Zugangsnummer | BC063281 | 
| Gene symbol | SREBF1 | 
| Gene ID (NCBI) | 6720 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125 kDa precursor form and the 68 kDa mature form of human SREBF1.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for SREBF1 antibody 14088-1-AP | Protokoll herunterladen | 
| IHC protocol for SREBF1 antibody 14088-1-AP | Protokoll herunterladenl | 
| IF protocol for SREBF1 antibody 14088-1-AP | Protokoll herunterladen | 
| IP protocol for SREBF1 antibody 14088-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Cancer Cell KMT2C deficiency drives transdifferentiation of double-negative prostate cancer and confer resistance to AR-targeted therapy | ||
| Cell Metab CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.
 | ||
| Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
| Nat Commun Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Isha (Verified Customer) (05-18-2022) | Antibody worked perfect at 1:1000 dilution for W.B. and 1:500 for IF. And for chIP 3ug/ml worked best. 
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