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SREBF1 Monoklonaler Antikörper

SREBF1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(59)

CloneNo.

1B6G5

Kat-Nr. : 66875-1-Ig

Synonyme

SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, bHLHd1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF-7-Zellen, A549-Zellen, HeLa-Zellen, HT-29-Zellen, Jurkat-Zellen, L02-Zellen, MOLT-4-Zellen
Erfolgreiche Detektion in IHChumanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:16000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66875-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA SREBF1 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Rind
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen SREBF1 fusion protein Ag5484
Vollständiger Name sterol regulatory element binding transcription factor 1
Berechnetes Molekulargewicht 1177 aa, 125 kDa
Beobachtetes Molekulargewicht 125 kDa
GenBank-ZugangsnummerBC063281
Gene symbol SREBF1
Gene ID (NCBI) 6720
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125kd precursor form and the 68kd mature form of human SREBF1.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SREBF1 antibody 66875-1-IgProtokoll herunterladen
IHC protocol for SREBF1 antibody 66875-1-IgProtokoll herunterladenl
IF protocol for SREBF1 antibody 66875-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai
humanWB

Nat Commun

Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease

Authors - Evangelia Lekka
WB,IF

Phytomedicine

Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis

Authors - Daofei Song
humanWB

Phytomedicine

Asperosaponin VI protects alcohol-induced hepatic steatosis and injury via regulating lipid metabolism and ER stress

Authors - Linlin Wei
human,WB

J Hazard Mater

In vitro effects of Triclocarban on adipogenesis in murine preadipocyte and human hepatocyte.

Authors - Manyuan Dong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Iram (Verified Customer) (08-14-2020)

we saw very clean staining of SREBF1 in our tissue sections.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
  • Cell Tissue Type: Human colon cancer cells
FH

Miray (Verified Customer) (11-25-2019)

it works well for the liver lysate isolated from mice liver.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Liver
SREBF1 Antibody Western Blot, validation (1:1000 dilution) in Liver (Cat no:66875-1-Ig)