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  • KD/KO Validated

SREBF2 Polyklonaler Antikörper

SREBF2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(67)

Kat-Nr. : 28212-1-AP

Synonyme

bHLHd2, Class D basic helix-loop-helix protein 2, Processed sterol regulatory element-binding protein 2, SREBF 2, SREBP 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHUVEC-Zellen, HeLa-Zellen, HepG2-Zellen, PC-3-Zellen
Erfolgreiche IPHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

28212-1-AP bindet in WB, IP, CoIP, ELISA SREBF2 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, hamster, Hausschwein, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SREBF2 fusion protein Ag28205
Vollständiger Name sterol regulatory element binding transcription factor 2
Berechnetes Molekulargewicht 124 kDa
Beobachtetes Molekulargewicht73-75 kDa, 120-130 kDa
GenBank-ZugangsnummerBC056158
Gene symbol SREBF2
Gene ID (NCBI) 6721
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues. SREBF2 exists two isoform with molecular weight 124 kDa and 73 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SREBF2 antibody 28212-1-APProtokoll herunterladen
IP protocol for SREBF2 antibody 28212-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nature

7-Dehydrocholesterol dictates ferroptosis sensitivity

Authors - Yaxu Li
HumanWB

Cancer Cell

Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis

Authors - Wei Zhou
humanWB

Redox Biol

Lipophagy-mediated cholesterol synthesis inhibition is required for the survival of hepatocellular carcinoma under glutamine deprivation

Authors - Youzi Kong
  • KD Validated
humanWB

Hypertension

Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia

Authors - Lunbo Tan
mouseWB

Acta Pharmacol Sin

Urolithin A promotes atherosclerotic plaque stability by limiting inflammation and hypercholesteremia in Apolipoprotein E-deficient mice

Authors - Meng-Yun Xu
humanWB

Mol Metab

CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

Authors - Han Zeng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Simon (Verified Customer) (07-23-2025)

The antibody works well for Jurkat cells, showin the precursor and the mature SREBF2

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: lymphoblasts (Jurkat cell line)
SREBF2 Antibody Western Blot validation (1:4000 dilution) in lymphoblasts (Jurkat cell line) (Cat no:28212-1-AP)
FH

Shunbin (Verified Customer) (05-14-2024)

Not good. Only non specific bands detected

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: B cell lines
SREBF2 Antibody Western Blot validation (1:1000 dilution) in B cell lines (Cat no:28212-1-AP)
FH

Shunbin (Verified Customer) (05-14-2024)

Many non specific bands, but no specific band can be identified

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
SREBF2 Antibody Western Blot validation (1:1000 dilution) in (Cat no:28212-1-AP)
FH

Bertrand (Verified Customer) (04-08-2024)

This is a Srebp2 antibody, however, the results from western blot analysis show that the product might not be specific to Srebf2...There are lots of non-specific bands and the uncleaved/immature protein should not be present in the nucleus of cells, yet it was there.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Liver homogenates
FH

Boyan (Verified Customer) (04-17-2023)

OK for WB, a specific but weak band was seen around 150 kd, but not 130 kd, which is shown on its booklet. The 130 kd band is a strong nonspecific band.

  • Applications: Western Blot