SRF Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SRF Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 16821-1-AP

Synonyme

MCM1, Serum response factor, SRF

---

Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 16821-1-AP

  WB result of SRF antibody (16821-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells.

• 

  WB analysis using 16821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16821-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16821-1-AP

  IP Result of anti-SRF (IP:16821-1-AP, 3ug; Detection:16821-1-AP 1:1000) with HeLa cells lysate 3000ug.

• 

  IHC staining of human brain using 16821-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16821-1-AP (SRF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16821-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16821-1-AP (SRF antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16821-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16821-1-AP (SRF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

16821-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA SRF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRF fusion protein Ag10386
• Vollständiger Name: serum response factor (c-fos serum response element-binding transcription factor)
• Berechnetes Molekulargewicht: 508 aa, 52 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa, 40 kDa
• GenBank-Zugangsnummer: BC048211
• Gene symbol: SRF
• Gene ID (NCBI): 6722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

Publikationen

Species	Application	Title
	WB	Cell Physiol Biochem; Galectin-3- Mediated Transdifferentiation of Pulmonary Artery Endothelial Cells Contributes to Hypoxic Pulmonary Vascular Remodeling.; Authors - Li Zhang
mouse	WB	Stem Cell Res Ther; Activin B-activated Cdc42 signaling plays a key role in regulating adipose-derived mesenchymal stem cells-mediated skin wound healing.; Authors - Simin Huang
mouse	ChIP	J Immunol; TPL-2 Inhibits IFN-β Expression via an ERK1/2-TCF-FOS Axis in TLR4-Stimulated Macrophages.; Authors - Louise Blair
mouse	WB	J Am Heart Assoc; Targeting AGGF1 (angiogenic factor with G patch and FHA domains 1) for Blocking Neointimal Formation After Vascular Injury.; Authors - Yufeng Yao
human	WB	Front Oncol; SRSF3 Promotes Angiogenesis in Colorectal Cancer by Splicing SRF.; Authors - Yinshuang Chen ; KD Validated
human	WB	J Cell Mol Med; microRNA let-7g suppresses PDGF-induced conversion of vascular smooth muscle cell into the synthetic phenotype.; Authors - Tzu-Ming Wang