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SRF Polyklonaler Antikörper
SRF Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16821-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A431-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | Mausherzgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | A431-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:2000-1:8000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 2 publications below |
WB | See 20 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
IP | See 3 publications below |
CoIP | See 3 publications below |
ChIP | See 2 publications below |
Produktinformation
16821-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA SRF und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | SRF fusion protein Ag10386 |
Vollständiger Name | serum response factor (c-fos serum response element-binding transcription factor) |
Berechnetes Molekulargewicht | 508 aa, 52 kDa |
Beobachtetes Molekulargewicht | 65-70 kDa, 40 kDa |
GenBank-Zugangsnummer | BC048211 |
Gene symbol | SRF |
Gene ID (NCBI) | 6722 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for SRF antibody 16821-1-AP | Protokoll herunterladen |
IHC protocol for SRF antibody 16821-1-AP | Protokoll herunterladenl |
IF protocol for SRF antibody 16821-1-AP | Protokoll herunterladen |
IP protocol for SRF antibody 16821-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Exp Mol Med Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer
| ||
Cell Mol Life Sci Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferation | ||
Am J Respir Cell Mol Biol SRF Expression in Excess Permits a Dual Contractile-Proliferative Phenotype of Airway Smooth Muscle | ||
Cell Physiol Biochem Galectin-3- Mediated Transdifferentiation of Pulmonary Artery Endothelial Cells Contributes to Hypoxic Pulmonary Vascular Remodeling. | ||
Stem Cell Res Ther Activin B-activated Cdc42 signaling plays a key role in regulating adipose-derived mesenchymal stem cells-mediated skin wound healing. | ||
Am J Physiol Lung Cell Mol Physiol Myocardin regulates fibronectin expression and secretion from human pleural mesothelial cells |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Carmen (Verified Customer) (11-29-2024) | Works well IHC set up on the Leica Bond
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