SRF Monoklonaler Antikörper

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• KD/KO Validated

SRF Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1G8F4

Kat-Nr. : 66742-1-Ig

Synonyme

1G8F4, MCM1, Serum response factor

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  WB analysis using 66742-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66742-1-Ig

  WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells.

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  WB analysis using 66742-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66742-1-Ig

  WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected HeLa cells.

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  WB analysis of RAW 264.7 using 66742-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66742-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SRF antibody (66742-1-Ig, Clone: 1G8F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66742-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66742-1-Ig (SRF antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

66742-1-Ig bindet in WB, IF/ICC, ELISA SRF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SRF fusion protein Ag10386
• Vollständiger Name: serum response factor (c-fos serum response element-binding transcription factor)
• Berechnetes Molekulargewicht: 508 aa, 52 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC048211
• Gene symbol: SRF
• Gene ID (NCBI): 6722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

Publikationen

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis; Authors - Ni Zeng
human	WB	bioRxiv; Single molecule imaging of the central dogma reveals myosin-2A gene expression is regulated by contextual translational buffering; Authors - O'Neil Wiggan ; KD Validated
mouse	WB	Mol Neurobiol; SRF Facilitates Transcriptional Inhibition of Gem Expression by m6A Methyltransferase METTL3 to Suppress Neuronal Damage in Epilepsy; Authors - Lianling Li