SRI Polyklonaler Antikörper

SRI Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16471-1-AP

Synonyme

22 kDa protein, CP 22, SCN, sorcin, SRI, V19

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 16471-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16471-1-AP (SRI Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16471-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16471-1-AP (SRI Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16471-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16471-1-AP (SRI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16471-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16471-1-AP (SRI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16471-1-AP

  IP Result of anti-SRI (IP:16471-1-AP, 3ug; Detection:16471-1-AP 1:500) with HepG2 cells lysate 600ug.

• 

  IHC staining of human liver using 16471-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16471-1-AP (SRI antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human liver using 16471-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16471-1-AP (SRI antibody) at dilution of 1:500 (under 40x lens).

• 

  IF Staining of HepG2 using 16471-1-AP

  Immunofluorescent analysis of HepG2 cells using 16471-1-AP (SRI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, humanes Lebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

16471-1-AP bindet in WB, IP, IHC, IF SRI und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRI fusion protein Ag9553
• Vollständiger Name: sorcin
• Berechnetes Molekulargewicht: 198 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC011025
• Gene symbol: SRI
• Gene ID (NCBI): 6717
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Cell Physiol; Targeting P-glycoprotein and SORCIN: Dihydromyricetin strengthens anti-proliferative efficiency of adriamycin via MAPK/ERK and Ca(2+) -mediated apoptosis pathways in MCF-7/ADR and K562/ADR.; Authors - Yaoting Sun
human	WB	J Cell Physiol; PMID: 30171603; Authors - Yaoting Sun
mouse	IF, WB	Arch Biochem Biophys; Knockdown of sorcin increases HEI-OC1 cell damage induced by cisplatin in vitro.; Authors - Dan Wang
human	WB,IHC,IF,IP	Cell Death Dis; AnnexinA7 promotes epithelial-mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma.; Authors - Fei Ling