SRX1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SRX1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(35)

Rezensionen (2)

Kat-Nr. : 14273-1-AP

Synonyme

C20orf139, SRX, SRX1, SRXN1, Sulfiredoxin 1, YKL086W

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  WB analysis using 14273-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14273-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14273-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14273-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 14273-1-AP

  IP result of anti-SRX1 (IP:14273-1-AP, 3ug; Detection:14273-1-AP 1:400) with A549 cells lysate 3300ug.

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  IHC staining of human lung cancer using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14273-1-AP (SRX1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14273-1-AP (SRX1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14273-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen, Mauslebergewebe, Transfizierte HEK-293-Zellen, U-251-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 27 publications below
• IHC: See 14 publications below
• IF: See 2 publications below

Produktinformation

14273-1-AP bindet in WB, IHC, IF, IP, ELISA SRX1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRX1 fusion protein Ag5613
• Vollständiger Name: sulfiredoxin 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC047707
• Gene symbol: SRXN1
• Gene ID (NCBI): 140809
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse		Nat Commun; Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle.; Authors - John T Olthoff
human	WB,IHC	Proc Natl Acad Sci U S A; Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.; Authors - Wei Qiou Q
human	IHC	Cancer Res; Genetic polymorphisms and protein expression of NRF2 and Sulfiredoxin predict survival outcomes in breast cancer.; Authors - Hartikainen Jaana M JM
human	WB	Oncogene; PMID: 28504720; Authors - E-J Choi
human,mouse	WB,IHC	Cancer Lett; Nrf2-activated expression of sulfiredoxin contributes to urethane-induced lung tumorigenesis.; Authors - Murli Mishra ; KD Validated
mouse	WB,IHC	Carcinogenesis; Tumor Promoter-induced Sulfiredoxin Is Required for Mouse Skin Tumorigenesis.; Authors - Lisha Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford (Verified Customer) (09-23-2023)

Detecting lower molecular weight molecules in gels is relatively difficult, we tried several antibodies from different companies and this worked well at a concentration of 1:500.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Breast cancer cells

SRX1 Antibody Western Blot validation (1:500 dilution) in Breast cancer cells (Cat no:14273-1-AP)

FH

Christopher (Verified Customer) (12-03-2018)

Primary neurons were lysed in RIPA and 20ug samples (containing 1x Laemmli) were run on Bis-Tris gels in 1xMOPS buffer. This antibody recognised SRXN-1 in HEK293FT cells transfected with Human SRXN1 at 17kDa. In HEK cells, non specific bands were visible at around 28 and 35kDa as well.In primary neurons, the antibody recognised a band at roughly 15kDa however it binds non sepcifically to the Pageruler Prestained protein ladder which is an issue.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Primary cortical neurons and transfected HEK293FT cells