SSB Polyklonaler Antikörper

SSB Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 11720-1-AP

Synonyme

La, La autoantigen, La ribonucleoprotein, LARP3, Lupus La protein

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  WB analysis of Jurkat using 11720-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11720-1-AP (SSB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11720-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11720-1-AP (SSB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11720-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11720-1-AP (SSB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 11720-1-AP

  IP result of anti-SSB (IP:11720-1-AP, 4ug; Detection:11720-1-AP 1:300) with Jurkat cells lysate 3600ug.

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  IF Staining of HeLa using 11720-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SSB antibody (11720-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

11720-1-AP bindet in WB, IF/ICC, IP, ELISA SSB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SSB fusion protein Ag2294
• Vollständiger Name: Sjogren syndrome antigen B (autoantigen La)
• Berechnetes Molekulargewicht: 408 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC001289
• Gene symbol: SSB
• Gene ID (NCBI): 6741
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	EMBO Rep; Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs.; Authors - Ahmed S Warda
human	WB	Oxid Med Cell Longev; The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress.; Authors - Jun Tian
human	WB	J Virol; Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatocyte Nuclear Factor 6.; Authors - Ruidong Hao
human	WB	Cell Mol Biol Lett; Salivary gland protective and antiinflammatory effects of genistein in Sjögren's syndrome by inhibiting Xist/ACSL4-mediated ferroptosis following binding to estrogen receptor-alpha; Authors - Tianjiao Mao