TRAPB/SSR2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRAPB/SSR2 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10278-1-AP

Synonyme

HSD25, SSR beta, SSR2, TLAP, TRAP BETA, TRAPB, TRAPB/SSR2

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Galerie der Validierungsdaten

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  WB analysis using 10278-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10278-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10278-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 10278-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10278-1-AP (TRAPB/SSR2 antibody at dilution of 1:50.

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  IHC staining of human pancreas using 10278-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10278-1-AP (TRAPB/SSR2 antibody at dilution of 1:50.

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  IF Staining of HepG2 using 10278-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRAPB/SSR2 antibody (10278-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 10278-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug TRAPB/SSR2 antibody (10278-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, L02-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Produktinformation

10278-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TRAPB/SSR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAPB/SSR2 fusion protein Ag0304
• Vollständiger Name: signal sequence receptor, beta (translocon-associated protein beta)
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC000341
• Gene symbol: SSR2
• Gene ID (NCBI): 6746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAPB (translocon-associated protein subunit beta), also known as SSR2 (signal sequence receptor subunit beta), is a 22-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 7789174). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

Publikationen

Species	Application	Title
human	WB	Mol Cell; RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.; Authors - Xianyan Hu
human	WB	Sci Adv; The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.; Authors - Chatchai Phoomak
human	WB	Diabetes; The Role of TRAPγ/SSR3 in Preproinsulin Translocation into the Endoplasmic Reticulum.; Authors - Xiaoxi Xu
rat	WB, IP	FASEB J; Deficient endoplasmic reticulum translocon-associated protein complex limits the biosynthesis of proinsulin and insulin.; Authors - Yumeng Huang ; KD Validated
human	WB,IHC	J Cell Mol Med; Overexpression of SSR2 promotes proliferation of liver cancer cells and predicts prognosis of patients with hepatocellular carcinoma.; Authors - Fengsui Chen ; KD Validated
human	IHC	J Immunol Res; Endoplasmic Reticulum Stress-Related Signature for Predicting Prognosis and Immune Features in Hepatocellular Carcinoma; Authors - Genhao Zhang