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  • KD/KO Validated

ST6GAL1 Polyklonaler Antikörper

ST6GAL1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 14355-1-AP

Synonyme

Alpha 2,6-ST 1, B-cell antigen CD75, Beta-galactoside alpha-2,6-sialyltransferase 1, CD75, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, HuH-7-Zellen, Jurkat-Zellen, L02-Zellen, Mausmilzgewebe, U-937-Zellen
Erfolgreiche IPRaji-Zellen
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe, humanes Hautgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14355-1-AP bindet in WB, IHC, IF, IP, ELISA ST6GAL1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ST6GAL1 fusion protein Ag5705
Vollständiger Name ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
Berechnetes Molekulargewicht 47 kDa
Beobachtetes Molekulargewicht 43-45 kDa, 50-70 kDa
GenBank-ZugangsnummerBC040009
Gene symbol ST6GAL1
Gene ID (NCBI) 6480
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST6GAL1 (β-galactoside α-2-6 sialyl transferase1; also known as ST6N or CD75) is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta, and skeletal muscle. ST6GAL1 undergoes proteolytic process to generate soluble form from membrane form. Western blot analysis of human liver using this antibody detects both isoforms between 43-50 kDa. Higher molecular weight of bands around 50-70 kDa can also be observed with glycosylation modification. (PMID: 15049997, 23358684)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ST6GAL1 antibody 14355-1-APProtokoll herunterladen
IHC protocol for ST6GAL1 antibody 14355-1-APProtokoll herunterladenl
IP protocol for ST6GAL1 antibody 14355-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouse,humanWB,IF

Immunity

Immune checkpoint therapy-elicited sialylation of IgG antibodies impairs antitumorigenic type I interferon responses in hepatocellular carcinoma

Authors - Rui-Qi Wu
mouse,humanWB,IHC

Int J Cancer

Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.

Authors - Qingmin Yuan
  • KD Validated
mouse,humanIHC,IF

Mol Cell Proteomics

Integrated Systems Analysis of the Murine and Human Pancreatic Cancer Glycomes Reveals a Tumor-Promoting Role for ST6GAL1

Authors - Emma Kurz
  • KO Validated
humanWB

Front Oncol

High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.

Authors - Jun Wang
  • KD Validated
humanWB

Am J Cancer Res

α1,6-Fucosyltransferase (FUT8) regulates the cancer-promoting capacity of cancer-associated fibroblasts (CAFs) by modifying EGFR core fucosylation (CF) in non-small cell lung cancer (NSCLC).

Authors - Fengzhou Li
ratWB

Int J Food Sci Nutr

Ellagic acid alleviates TNBS-induced intestinal barrier dysfunction by regulating mucin secretion and maintaining tight junction integrity in rats

Authors - Bo Peng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boyan (Verified Customer) (04-23-2024)

The strong band around 60 kd is a non-specific band. 1) This band doesn't respond to RNAi (I have another St6gal1 antibody which can confirm the RNAi was working), and 2) this band doesn't respond to PNGase deglycosylation (St6gal1 carries glycosylation, which should show a downshift after PNGase treatment).

  • Applications: Western Blot
FH

Vanessa (Verified Customer) (01-12-2024)

1:1000 dilution of the primary antibody accompanied by the recommended blocking, washing and incubation steps resulted in crisp bands with no background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Jurkat cells
FH

Maggie (Verified Customer) (11-15-2023)

Overnight incubation

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: PC3 cells
ST6GAL1 Antibody Immunofluorescence validation (1:200 dilution) in PC3 cells (Cat no:14355-1-AP)
FH

Faezeh (Verified Customer) (01-29-2023)

It showed two bands in A549 and PANC1 cell lines, we validated that top band is the correct one using siRNA against ST6GAL1.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
ST6GAL1 Antibody Western Blot validation (1:1000 dilution) in (Cat no:14355-1-AP)
FH

Dawn (Verified Customer) (02-22-2022)

It is difficult to find good antibodies for glycoslytransferase genes, but this is a good one. It works in multiple cell lines as well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: A549 cells - lysate after miRNA transfection.
ST6GAL1 Antibody Western Blot validation (1:1000 dilution) in A549 cells - lysate after miRNA transfection. (Cat no:14355-1-AP)
FH

Patricia (Verified Customer) (02-25-2020)

Did not work- even for positive control

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovarian cancer