STEAP4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

STEAP4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

32

Rezensionen (1)

Kat-Nr. : 11944-1-AP

Synonyme

Metalloreductase STEAP4, STAMP2, STEAP family member 4, STEAP4, TIARP, TNFAIP9

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Galerie der Validierungsdaten

• 

  WB analysis using 11944-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 11944-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11944-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11944-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11944-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 11944-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of 3T3-L1 using 11944-1-AP

  IP result of anti-STEAP4 (IP:11944-1-AP, 4ug; Detection:11944-1-AP 1:1000) with 3T3-L1 cells lysate 1200 ug.

• 

  IP experiment of 3T3-L1 using 11944-1-AP

  IP result of anti-STEAP4(IP:11944-1-AP, 4ug; Detection:11944-1-AP 1:500) with 3T3-L1 cells lysate 1360 ug.

• 

  IHC staining of human prostate cancer using 11944-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11944-1-AP (STEAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 3T3-L1-Zellen, humanes Hirngewebe, humanes Lebergewebe, Rattenlebergewebe, RAW 264.7-Zellen
• Erfolgreiche IP: 3T3-L1-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 25 publications below
• IHC: See 16 publications below
• IF: See 2 publications below

Produktinformation

11944-1-AP bindet in WB, IP, IHC, IF, ELISA STEAP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STEAP4 fusion protein Ag2545
• Vollständiger Name: STEAP family member 4
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC020600
• Gene symbol: STEAP4
• Gene ID (NCBI): 79689
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STEAP4 (also called STAMP2), is a member of the STEAP family. Structurally, It is a 459 amino acid protein characterized by a molecular topology of six transmembrane domains. The cytoplasmic N-terminal domain of STEAP4 shows structural similarity with bacterial and archaeal FNO oxidoreductase and STEAP4 exhibits a strong iron reductase activity. Studies in mice and human suggest that STEAP4 maybe involved in adipocyte development and metabolism, and may contribute to the normal biology of the prostate cell, as well as prostate cancer progression.

Publikationen

Species	Application	Title
human	IHC	J Allergy Clin Immunol; Six-transmembrane epithelial antigens of the prostate comprise a novel inflammatory nexus in patients with pustular skin disorders.; Authors - Yun Liang
mouse,rat	WB,IHC	Bone Res; Dietary fat-associated osteoarthritic chondrocytes gain resistance to lipotoxicity through PKCK2/STAMP2/FSP27.; Authors - Sung Won Lee ; KD Validated
mouse,human,rat	IHC,WB	Basic Res Cardiol; The six-transmembrane protein Stamp2 ameliorates pulmonary vascular remodeling and pulmonary hypertension in mice.; Authors - Mehreen Batool ; KO Validated
human	WB,IHC	Nat Commun; Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis.; Authors - Yun Liao
mouse	IF	Proc Natl Acad Sci U S A; Quantitative proteomics identifies STEAP4 as a critical regulator of mitochondrial dysfunction linking inflammation and colon cancer.; Authors - Xiang Xue
human, mouse	WB, IHC	J Hepatol; PMID: 25646886; Authors - Hye Y Kim ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiang (Verified Customer) (11-08-2019)

Works pretty good.

• Applications: Western Blot, Immunofluorescence,

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