STIL Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

STIL Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1E12C1

Kat-Nr. : 66876-1-Ig

Synonyme

1E12C1, SCL-interrupting locus protein, SIL, TAL-1-interrupting locus protein

---

• 

  WB analysis using 66876-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66876-1-Ig (STIL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 66876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 66876-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using STIL antibody (66876-1-Ig, Clone: 1E12C1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  IF Staining of Jurkat using 66876-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using STIL antibody (66876-1-Ig, Clone: 1E12C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Daudi-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66876-1-Ig bindet in WB, IHC, IF/ICC, ELISA STIL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STIL fusion protein Ag19124
• Vollständiger Name: SCL/TAL1 interrupting locus
• Berechnetes Molekulargewicht: 1287 aa, 143 kDa
• Beobachtetes Molekulargewicht: 150-155 kDa
• GenBank-Zugangsnummer: BC126223
• Gene symbol: STIL
• Gene ID (NCBI): 6491
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Cell Int; ncRNAs-mediated overexpression of STIL predict unfavorable prognosis and correlated with the efficacy of immunotherapy of hepatocellular carcinoma; Authors - Longwen Xu
human	WB	Cancer Cell Int; The biological function and clinical significance of STIL in osteosarcoma.; Authors - Shu-Fan Ji ; KD Validated
human	WB	J Cell Physiol; Primary cilium participates in radiation-induced bystander effects through TGF-β1 signaling; Authors - Pei Qu ; KD Validated
human	IF	Cell Rep; Dual roles of CCDC102A in governing centrosome duplication and cohesion; Authors - Tianning Wang