STIM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

STIM1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(69)

Rezensionen (2)

Kat-Nr. : 11565-1-AP

Synonyme

GOK, IMD10, STIM 1, stim-1, STRMK

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  WB analysis using 11565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11565-1-AP (STIM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11565-1-AP

  IP result of anti-STIM1 (IP:11565-1-AP, 4ug; Detection:11565-1-AP 1:20000) with HeLa cells lysate 1400 ug.

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  IHC staining of mouse lung using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11565-1-AP (STIM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11565-1-AP (STIM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 11565-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using STIM1 antibody (11565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 11565-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human STIM1 (11565-1-AP)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 15 publications below
• WB: See 51 publications below
• IHC: See 8 publications below
• IF: See 20 publications below
• IP: See 3 publications below

Produktinformation

11565-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA STIM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STIM1 fusion protein Ag2140
• Vollständiger Name: stromal interaction molecule 1
• Berechnetes Molekulargewicht: 685 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77-90 kDa
• GenBank-Zugangsnummer: BC021300
• Gene symbol: STIM1
• Gene ID (NCBI): 6786
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.; Authors - Ao Hu ; KO Validated
human	IF	J Immunother Cancer; PD1 blockade enhances K+ channel activity, Ca2+ signaling, and migratory ability in cytotoxic T lymphocytes of patients with head and neck cancer.; Authors - Hannah S Newton
mouse	WB,IF	Proc Natl Acad Sci U S A; STIM1-Ca2+ signaling modulates automaticity of the mouse sinoatrial node.; Authors - Hengtao Zhang
mouse,human	WB	Cell Mol Life Sci; STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons; Authors - Karolina Serwach ; KD Validated
Mouse		Mol Metab; Disruption of STIM1-mediated Ca2+ sensing and energy metabolism in adult skeletal muscle compromises exercise tolerance, proteostasis and lean mass.; Authors - Rebecca J Wilson
human	WB,IF	Int J Mol Sci; Expression and Localization of Thrombospondins, Plastin 3, and STIM1 in Different Cartilage Compartments of the Osteoarthritic Varus Knee.; Authors - Daniela Mählich

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:1000

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma cells

FH

lieqi (Verified Customer) (12-19-2018)

• Applications: Western Blot,
• Cell Tissue Type: T84

STIM1 Antibody Western Blot, validation ( dilution) in T84 (Cat no:11565-1-AP)