STK39 Rekombinanter Antikörper

STK39 Rekombinant Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230382G9

Kat-Nr. : CL488-83081-2

Synonyme

230382G9, DCHT, PASK, SPAK, Ste 20 related kinase

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  IF Staining of mouse brain using CL488-83081-2

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 STK39 antibody (CL488-83081-2, Clone: 230382G9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using CL488-83081-2

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 STK39 antibody (CL488-83081-2, Clone: 230382G9 ) at dilution of 1:200.

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  FC experiment of Daudi using CL488-83081-2

  1x10^6 Daudi cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Stk39 Recombinant Antibody (CL488-83081-2, Clone:230382G9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): Daudi-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-83081-2 bindet in IF/ICC, IF-P STK39 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: STK39 fusion protein Ag34503
• Vollständiger Name: serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC166614
• Gene symbol: STK39
• Gene ID (NCBI): 27347
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).