STRAP Polyklonaler Antikörper

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• KD/KO Validated

STRAP Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 18277-1-AP

Synonyme

MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD

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Galerie der Validierungsdaten

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  WB analysis using 18277-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18277-1-AP (STRAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18277-1-AP

  WB result of STRAP antibody (18277-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STRAP transfected HEK-293 cells.

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  IP experiment of HeLa using 18277-1-AP

  IP Result of anti-STRAP (IP:18277-1-AP, 4ug; Detection:18277-1-AP 1:2000) with HeLa cells lysate 2000ug.

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  IHC staining of human ovary tumor using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18277-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STRAP antibody (18277-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

18277-1-AP bindet in WB, IP, IHC, IF, ELISA STRAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STRAP fusion protein Ag13085
• Vollständiger Name: serine/threonine kinase receptor associated protein
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC000162
• Gene symbol: STRAP
• Gene ID (NCBI): 11171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.

Publikationen

Species	Application	Title
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy ; KD Validated
human	WB,IP	EMBO J; The p53-inducible long noncoding RNA TRINGS protects cancer cells from necrosis under glucose starvation.; Authors - Muhammad Riaz Khan ; KD Validated
human		Cancers (Basel); Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Knockout Decreases the Malignant Phenotype in Neuroblastoma Cell Lines.; Authors - Laura V Bownes ; KD Validated
human	WB	Front Oncol; STRAP as a New Therapeutic Target for Poor Prognosis of Pancreatic Ductal Adenocarcinoma Patients Mainly Caused by TP53 Mutation.; Authors - Shanshan Hu ; KD Validated
human	WB	J Biol Chem; The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells.; Authors - Evan P Booy
human	WB,IF	Int J Mol Sci; SIRT7 Deacetylates STRAP to Regulate p53 Activity and Stability.; Authors - Miao Yu

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