SUB1 Polyklonaler Antikörper

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• KD/KO Validated

SUB1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10948-2-AP

Synonyme

p14, PC4, Positive cofactor 4, RPO2TC1, SUB1, SUB1 homolog, SUB1 homolog (S. cerevisiae)

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Galerie der Validierungsdaten

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  WB analysis of A549 using 10948-2-AP

  WB result of SUB1 antibody (10948-2-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUB1 transfected A549 cells.

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  WB analysis of HeLa using 10948-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10948-2-AP

  WB result of SUB1 antibody (10948-2-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUB1 transfected HeLa cells.

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  WB analysis using 10948-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10948-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10948-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10948-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10948-2-AP (SUB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10948-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10948-2-AP (SUB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10948-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10948-2-AP (SUB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10948-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10948-2-AP (SUB1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 10948-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10948-2-AP (SUB1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10948-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10948-2-AP (SUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

10948-2-AP bindet in WB, IHC, IF, ELISA SUB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUB1 fusion protein Ag1390
• Vollständiger Name: SUB1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 18-23 kDa
• GenBank-Zugangsnummer: BC009610
• Gene symbol: SUB1
• Gene ID (NCBI): 10923
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SUB1 gene encodes activated RNA polymerase II transcriptional coactivator p15, also termed as positive cofactor 4 (PC4) or p14. SUB1 is a sort of general coactivator that functions in assembling and stablization of transcription complex. It has DNA binding activity and is localized in the nucleus, mulitple modification sites of phosphorylation or acetylation may turn it appear slightly shifted molecular weight. Catalog#10948-2-AP is a rabbit polyclonal antibody raised against full-length human SUB1. This antibody recognize 14-16 kDa (P14 or P15), 20 kDa(phospho form) and 30 kDa (Dimer form).

Publikationen

Species	Application	Title
mouse	WB,IHC	J Proteome Res; Novel breast cancer metastasis-associated proteins.; Authors - Ho Jiapei J
mouse	WB	J Cell Biochem; Knockdown of nucleosome assembly protein 1-like 1 promotes dimethyl sulfoxide-induced differentiation of P19CL6 cells into cardiomyocytes.; Authors - Li Lu L