SUCLG1 Polyklonaler Antikörper

SUCLG1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14923-1-AP

Synonyme

G ALPHA, SCS alpha, SUCLA1, SUCLG1

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Galerie der Validierungsdaten

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  WB analysis using 14923-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14923-1-AP (SUCLG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 14923-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14923-1-AP (SUCLG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 14923-1-AP

  IP result of anti-SUCLG1 (IP:14923-1-AP, 4ug; Detection:14923-1-AP 1:500) with mouse kidney tissue lysate 2800 ug.

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  IHC staining of human liver cancer using 14923-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14923-1-AP (SUCLG1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse kidney using 14923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Rattenlebergewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

14923-1-AP bindet in WB, IP, IHC, ELISA SUCLG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUCLG1 fusion protein Ag6481
• Vollständiger Name: succinate-CoA ligase, alpha subunit
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC000504
• Gene symbol: SUCLG1
• Gene ID (NCBI): 8802
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SUCLG1(succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial) is also named as SCS-alpha and belongs to the succinate/malate CoA ligase alpha subunit family. The SUCLG1 gene encodes a 35 kDa protein with a 40 amino acids transit peptide. This enzyme catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Defects in SUCLG1 are the cause of mitochondrial DNA depletion syndrome type 9 (MTDPS9).

Publikationen

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB	Mol Cell; Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation; Authors - Sophie Trefely
mouse	WB	Nat Commun; A genetically encoded fluorescent biosensor for detecting itaconate with subcellular resolution in living macrophages; Authors - Pengkai Sun
mouse	WB,IP	J Mol Cell Cardiol; Mitochondrial protein hyperacetylation underpins heart failure with preserved ejection fraction in mice.; Authors - Xin Liu