SULT1A1 Polyklonaler Antikörper

SULT1A1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 10911-2-AP

Synonyme

EC:2.8.2.1, HAST1/HAST2, OK/SW-cl.88, P PST, P PST 1

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  WB analysis of mouse liver using 10911-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10911-2-AP (SULT1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10911-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10911-2-AP (SULT1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10911-2-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10911-2-AP (SULT1A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10911-2-AP

  IP result of anti-SULT1A1 (IP:10911-2-AP, 3ug; Detection:10911-2-AP 1:1000) with mouse liver tissue lysate 12500ug.

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  IHC staining of human ovary cancer using 10911-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10911-2-AP (SULT1A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10911-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SULT1A1 antibody (10911-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HepG2 using 10911-2-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug SULT1A1 Polyclonal antibody (10911-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Mauslebergewebe, U-937-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 4 publications below

Produktinformation

10911-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA SULT1A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SULT1A1 fusion protein Ag1344
• Vollständiger Name: sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000923
• Gene symbol: SULT1A1
• Gene ID (NCBI): 6817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SULT1A1, also named as ST1A1, P-PST 1, ST1A3, Ts-PST, P-PST 1, STP and STP1, belongs to the sulfotransferase 1 family. It catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. SULT1A1 is also responsible for the sulfation and activation of minoxidil. It mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity.

Publikationen

Species	Application	Title
human	WB	Oxid Med Cell Longev; Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells.; Authors - Xu Zheng
rat,human	WB,IHC	Biochem Pharmacol; Identification of metabolic pattern and bioactive form of resveratrol in human medulloblastoma cells.; Authors - Shu Xiao-Hong XH
human	WB	FEBS J; Distinct sulfonation activities in resveratrol-sensitive and resveratrol-insensitive human glioblastoma cells.; Authors - Sun Zheng Z
mouse	WB	Drug Metab Dispos; Sulfotransferase 4A1 (SULT4A1) increases its expression in mouse neurons as they mature.; Authors - Takuyu Hashiguchi
Human	IHC	J Gastroenterol; Differential gene expression in normal esophagus and Barrett's esophagus.; Authors - Jacob Wang
human	WB,IHC	PLoS One; Metabolic patterns and biotransformation activities of resveratrol in human glioblastoma cells: relevance with therapeutic efficacies.; Authors - Shu Xiao-Hong XH