SULT1A1 Monoklonaler Antikörper

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• KD/KO Validated

SULT1A1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

10H6F6

Kat-Nr. : 60076-1-Ig

Synonyme

EC:2.8.2.1, HAST1/HAST2, OK/SW-cl.88, P PST, P PST 1

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  WB analysis using 60076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60076-1-Ig

  WB result of SULT1A1 antibody (60076-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SULT1A1 transfected Jurkat cells.

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  WB analysis using 60076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 60076-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 60076-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, HEK-293-Zellen, HepG2-Zellen, humanes Lebergewebe, humanes Plazenta-Gewebe, Jurkat-Zellen, Maushirngewebe, Mauslebergewebe, Rattenhirngewebe, Rattenlebergewebe, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

60076-1-Ig bindet in WB, ELISA SULT1A1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SULT1A1 fusion protein Ag4611
• Vollständiger Name: sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000923
• Gene symbol: SULT1A1
• Gene ID (NCBI): 6817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SULT1A1, also named as ST1A1, P-PST 1, ST1A3, Ts-PST, P-PST 1, STP and STP1, belongs to the sulfotransferase 1 family. It catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. SULT1A1 is also responsible for the sulfation and activation of minoxidil. It mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity.

Publikationen

Species	Application	Title
rat	WB	J Chromatogr B Analyt Technol Biomed Life Sci; Liquid chromatography coupled with high resolution mass spectrometry reveals the inhibitory effects of Huangkuisiwu formula on biosynthesis of protein-binding uremic toxins in rats with chronic kidney disease; Authors - Jianping Li