SUOX Polyklonaler Antikörper

SUOX Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC,ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15075-1-AP

Synonyme

sulfite oxidase, Sulfite oxidase, mitochondrial, SUOX

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Galerie der Validierungsdaten

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  WB analysis of human liver using 15075-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15075-1-AP (SUOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15075-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15075-1-AP (SUOX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15075-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15075-1-AP (SUOX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15075-1-AP

  IP result of anti-SUOX (IP:15075-1-AP, 4ug; Detection:15075-1-AP 1:1200) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human liver using 15075-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15075-1-AP (SUOX antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15075-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15075-1-AP (SUOX antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 15075-1-AP

  Immunofluorescent analysis of Hela cells, using SUOX antibody 15075-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15075-1-AP

  1X10^6 HeLa cells were stained with 0.2ug SUOX antibody (15075-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, L02-Zellen, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

15075-1-AP bindet in WB, IP, IHC, IF, FC,ELISA SUOX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUOX fusion protein Ag7146
• Vollständiger Name: sulfite oxidase
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 52 kDa, 60 kDa
• GenBank-Zugangsnummer: BC065193
• Gene symbol: SUOX
• Gene ID (NCBI): 6821
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SUOX(sulfite oxidase) catalyzes the oxidation of sulfite to sulfate, the terminal reaction in the oxidative degradation pathway of the sulfur-containing amino acids cysteine and methionine. The enzyme is a dimer of identical subunits and is located in the intermembrane space of mitochondria(PMID:9600976). Defects in SUOX are the cause of isolated sulfite oxidase deficiency (ISOD).

Publikationen

Species	Application	Title
mouse	WB	EMBO Mol Med; CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome.; Authors - Marta Luna-Sánchez
mouse	WB	Antioxid Redox Signal; The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS(-) Production and Evidence of Pancreatic and Lung Toxicity.; Authors - Roman Heather B HB
mouse,human	WB	Hum Mol Genet; Coenzyme Q10 modulates sulfide metabolism and links the mitochondrial respiratory chain to pathways associated to one carbon metabolism.; Authors - Pilar González-García
mouse	WB	Toxicol Lett; Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo.; Authors - Zhao Xiaoming X