SUZ12 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SUZ12 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Rezensionen (1)

Kat-Nr. : 20366-1-AP

Synonyme

ChET 9 protein, CHET9, JJAZ1, Joined to JAZF1 protein, KIAA0160, Polycomb protein SUZ12, SUZ12

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  WB analysis using 20366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20366-1-AP (SUZ12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20366-1-AP

  WB result of SUZ12 antibody (20366-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUZ12 transfected HEK-293 cells.

• 

  IP experiment of HeLa using 20366-1-AP

  IP result of anti-SUZ12 (IP:20366-1-AP, 4ug; Detection:20366-1-AP 1:500) with HeLa cells lysate 3200ug.

• 

  IP experiment of HeLa using 20366-1-AP

  IP result of anti-SUZ12 (IP:20366-1-AP, 4ug; Detection:20366-1-AP 1:500) with HeLa cells lysate 3200ug.

• 

  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:400.

• 

  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:100.

• 

  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:100.

• 

  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:200.

• 

  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:200.

• 

  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:400.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

20366-1-AP bindet in WB, IHC, IP, CoIP, ELISA SUZ12 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: suppressor of zeste 12 homolog (Drosophila)
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: NM_015355
• Gene symbol: SUZ12
• Gene ID (NCBI): 23512
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.

Publikationen

Species	Application	Title
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human	WB	Mol Cell; TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways.; Authors - Iva A Tchasovnikarova ; KD Validated
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang
human	WB	Eur J Med Chem; Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.; Authors - Cheng Wang
human	WB	Sci Rep; Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.; Authors - Masamitsu N Asaka
human	WB	Am J Cancer Res; N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex.; Authors - Jing Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nikhil (Verified Customer) (01-07-2022)

HCT116 whole cell extract, 20micro/lane. Lane 2-4 transient expression of EZH2

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116

SUZ12 Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:20366-1-AP)