SYAP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SYAP1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16272-1-AP

Synonyme

PRO3113, SYAP1, Synapse associated protein 1

---

Galerie der Validierungsdaten

• 

  WB analysis using 16272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 16272-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16272-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 16272-1-AP

  IP Result of anti-SYAP1 (IP:16272-1-AP, 4ug; Detection:16272-1-AP 1:500) with L02 cells lysate 1800ug.

• 

  IHC staining of human cervical cancer using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16272-1-AP (SYAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16272-1-AP (SYAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16272-1-AP (SYAP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16272-1-AP (SYAP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16272-1-AP

  Immunofluorescent analysis of HepG2 cells using 16272-1-AP (SYAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SYAP1 antibody (16272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 16272-1-AP

  Immunofluorescent analysis of A431 cells using 16272-1-AP (SYAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HepG2-Zellen, L02-Zellen, Mauslebergewebe, Mauslungengewebe, Rattenlungengewebe, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

16272-1-AP bindet in WB, IHC, IF, ELISA SYAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SYAP1 fusion protein Ag9342
• Vollständiger Name: synapse associated protein 1, SAP47 homolog (Drosophila)
• Berechnetes Molekulargewicht: 352 aa, 40 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC014657
• Gene symbol: SYAP1
• Gene ID (NCBI): 94056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.

Publikationen

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
mouse	WB,IF	Histochem Cell Biol; Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons.; Authors - Dominique Schmitt ; KO Validated
mouse	IF	Biol Open; An essential role of the mouse synapse-associated protein Syap1 in circuits for spontaneous motor activity and rotarod balance.; Authors - Cora R von Collenberg