SYK Polyklonaler Antikörper

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SYK Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 14858-1-AP

Synonyme

EC:2.7.10.2, IMD82, p72-Syk, spleen tyrosine kinase, Tyrosine protein kinase SYK

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  WB analysis of Raji using 14858-1-AP

  WB result of SYK antibody (14858-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SYK transfected Raji cells.

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  WB analysis using 14858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14858-1-AP (SYK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14858-1-AP (SYK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Ramos using 14858-1-AP

  1x10^6 Ramos cells were intracellularly stained with 0.4 ug SYK Polyclonal antibody (14858-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Appendizitis-Gewebe, humanes Nierengewebe, humanes Eierstockgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below

Produktinformation

14858-1-AP bindet in WB, IHC, FC (Intra), ELISA SYK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SYK fusion protein Ag6657
• Vollständiger Name: spleen tyrosine kinase
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 66-72 kDa
• GenBank-Zugangsnummer: BC001645
• Gene symbol: SYK
• Gene ID (NCBI): 6850
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYK belongs to the protein kinase superfamily, Tyr protein kinase family and SYK/ZAP-70 subfamily. It is positive effector of BCR-stimulated responses. SYK couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway It phosphorylates USP25 and regulates its intracellular levels. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660) SYK can be detected 66-72 kDa in human and mouse (PMID: 37661351).

Publikationen

Species	Application	Title
mouse	WB	Food Funct; Synergistic effects of L-theanine and epigallocatechin gallate in alleviating ovalbumin allergy by regulating intestinal immunity through inhibition of mast cell degranulation; Authors - Wei Xu
mouse	WB	Int Immunopharmacol; SAP130 released by ferroptosis tubular epithelial cells promotes macrophage polarization via Mincle signaling in sepsis acute kidney injury; Authors - Jing Zhang
mouse	IHC	J Ethnopharmacol; Yiqi Kaimi prescription regulates protein phosphorylation to promote intestinal motility in slow transit constipation; Authors - Yi-Bo Yao
mouse	WB	Brain Res; Inhibition of TREM-1 alleviates neuroinflammation by modulating microglial polarization via SYK/p38MAPK signaling pathway after traumatic brain injury; Authors - Tianqi Zhao
mouse	WB	Int J Biol Macromol; A nanobody-enzyme fusion protein targeting PD-L1 and sialic acid exerts anti-tumor effects by C-type lectin pathway-mediated tumor associated macrophages repolarizing; Authors - Yongliang Tong
mouse	WB	Res Vet Sci; TYROBP modulates NFκB phosphorylation and SQSTM1 expression in macrophages during Brucella Abortus LPS infection; Authors - Wenping Wu