Nesprin 2 Polyklonaler Antikörper

Nesprin 2 Polyklonal Antikörper für WB, IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (2)

Kat-Nr. : 25265-1-AP

Synonyme

SYNE2, SYNE 2, NUANCE, NUA

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  WB analysis of HEK-293 using 25265-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25265-1-AP (SYNE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 25265-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 25265-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 25265-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Human dorsal cerebral organoid using 25265-1-AP

  Human dorsal cerebral organoid at 12 weeks. 4% PFA fixed. Antibody dilution was 1:500 for Syne-2 (25265-1-AP; white), DAPI 1:1000. Image created by Wimmer Ryszard from the Institut Curie in Paris, France

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IF: Human dorsal cerebral organoid, Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

25265-1-AP bindet in WB, IF, ELISA Nesprin 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: spectrin repeat containing, nuclear envelope 2
• Berechnetes Molekulargewicht: 799 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: NM_015180
• Gene symbol: Nesprin 2
• Gene ID (NCBI): 23224
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nesprin-2 is a member of actin-binding proteins that localize to the outer nuclear membrane. As a nuclear envelope component it provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Nesprin genes generate, through alternative splicing and alternative promoter and/or terminator usage, a multitude of differentially expressed isoforms, differing in domain architecture and length. The largest protein isoform, known as NUANCE or GIANT, has a predicted MW around 796 kDa. Other isoforms range from 30 kDa to 119 kDa.

Publikationen

Species	Application	Title
human	WB	Cancer Lett; Oncogenic functions and therapeutic potentials of targeted inhibition of SMARCAL1 in small cell lung cancer; Authors - Bei-Bei Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ryszard (Verified Customer) (09-02-2025)

Ventricular-like zone of a human brain dorsal organoid stained for Nesprin-2, imaged on an AXR Nikon Confocal Microscope 100x - max projection

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Cerebral Dorsal Organoid

Nesprin 2 Antibody Immunofluorescence validation (1:500 dilution) in Cerebral Dorsal Organoid (Cat no:25265-1-AP)

FH

Ryszard (Verified Customer) (07-25-2022)

Very nice staining with the Syne-2 antibody. I tried on Human Fetal Brain Tissue, Human Cerebral Organoids and Human Neural Stem Cells. They are very nice. Images attached to this comment from a Human Cerebral Organoid (Staining of Nesprin-2 in white LUT)

• Applications: Immunohistochemistry, Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Cerebral Organoids/Human Fetal Brain Tissue and Neural Stem Cells

Nesprin 2 Antibody Immunohistochemistry,Immunofluorescence,Cell culture validation (1:500 dilution) in Human Cerebral Organoids/Human Fetal Brain Tissue and Neural Stem Cells (Cat no:25265-1-AP)