SYNPO Polyklonaler Antikörper

SYNPO Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 29662-1-AP

Synonyme

KIAA1029, synaptopodin, SYNPO

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  WB analysis using 29662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29662-1-AP (SYNPO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 29662-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SYNPO antibody (29662-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 29662-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SYNPO antibody (29662-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

29662-1-AP bindet in WB, IF/ICC, ELISA SYNPO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SYNPO fusion protein Ag31287
• Vollständiger Name: synaptopodin
• Berechnetes Molekulargewicht: 929 aa, 99 kDa
• Beobachtetes Molekulargewicht: 44 kDa, 74-100 kDa
• GenBank-Zugangsnummer: BC146665
• Gene symbol: SYNPO
• Gene ID (NCBI): 11346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

Publikationen

Species	Application	Title
mouse	WB	Arch Physiol Biochem; Huaiqihuang (HQH) protects podocytes from high glucose-induced apoptosis and inflammation response by regulating PI3K/AKT/mTOR pathway; Authors - Peipei Zhang