SYNPO Monoklonaler Antikörper

SYNPO Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1E1B1

Kat-Nr. : 67339-1-Ig

Synonyme

Synaptopodin, 1E1B1, KIAA1029, snaptopodin

---

• 

  WB analysis of rat brain using 67339-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67339-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67339-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 67339-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67339-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 67339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 67339-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse kidney using 67339-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 67339-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Maushirngewebe, Hausschwein-Hirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe, Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:750-1:3000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

67339-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA SYNPO und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SYNPO fusion protein Ag15089
• Vollständiger Name: synaptopodin
• Berechnetes Molekulargewicht: 929 aa, 99 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC146665
• Gene symbol: SYNPO
• Gene ID (NCBI): 11346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

Publikationen

Species	Application	Title
mouse	WB	Front Cell Dev Biol; Umbilical Cord Mesenchymal Stem Cells Ameliorate Kidney Injury in MRL/Ipr Mice Through the TGF-β1 Pathway.; Authors - Chunkai Huang
mouse	WB	Cell Death Dis; Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease.; Authors - Shan Song
mouse	IF	Inflammation; Canagliflozin Attenuates Podocyte Inflammatory Injury through Suppressing the TXNIP/NLRP3 Signaling Pathway in Diabetic Kidney Disease Mice; Authors - Siyu Li