Synaptotagmin-1 Polyklonaler Antikörper

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• KD/KO Validated

Synaptotagmin-1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 14511-1-AP

Synonyme

P65, SVP65, Synaptotagmin 1, synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI

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Galerie der Validierungsdaten

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  WB analysis of HCT 116 using 14511-1-AP

  HCT 116 (WT and SYT1 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 14511-1-AP incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis using 14511-1-AP

  mouse cerebellum tissue was subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14511-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 14511-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14511-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HCT 116 using 14511-1-AP

  HCT 116 lysates prepared and IP of SYT1 performed using 1.0 μg of 14511-1-AP coupled to protein A- Sepharose beads. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of mouse testis using 14511-1-AP

  IP Result of anti-Synaptotagmin-1 (IP:14511-1-AP, 4ug; Detection:14511-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human liver cancer using 14511-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 14511-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HCT 116 using 14511-1-AP

  HCT 116 WT cells (yellow outline) and SYT1 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 14511-1-AP at 1:500 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IF Staining of HepG2 using 14511-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, HCT 116-Zellen, humanes Lebergewebe, Maushodengewebe, Y79-Zellen
• Erfolgreiche IP: Maushodengewebe, HCT 116-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HCT 116-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:4000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

14511-1-AP bindet in WB, IP, IHC, IF, ELISA Synaptotagmin-1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Synaptotagmin-1 fusion protein Ag5970
• Vollständiger Name: synaptotagmin I
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 65 kDa, 47 kDa
• GenBank-Zugangsnummer: BC058917
• Gene symbol: SYT1
• Gene ID (NCBI): 6857
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.

Publikationen

Species	Application	Title
human	WB	Cell Rep; The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus; Authors - Lang Wen
human	WB	Theranostics; ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells.; Authors - Tianhui Pan
		Front Mol Neurosci; A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13.; Authors - Yun Li
mouse	IHC,WB	Biochim Biophys Acta Mol Cell Biol Lipids; Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet.; Authors - Sara Sancho-Knapik
mouse	WB	J Proteomics; Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.; Authors - Hao-Long Zeng
rat	WB, IF	PLoS One; Identification of Protein Network Alterations upon Retinal Ischemia-Reperfusion Injury by Quantitative Proteomics Using a Rattus norvegicus Model.; Authors - Han Tian