Synaptotagmin-12 Polyklonaler Antikörper

Synaptotagmin-12 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 55015-1-AP

Synonyme

SRG1, Synaptotagmin 12, synaptotagmin XII, Synaptotagmin-12, SYT12, SytXII

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  WB analysis of mouse brain using 55015-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55015-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 55015-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 55015-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

55015-1-AP bindet in WB, IF, ELISA Synaptotagmin-12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: synaptotagmin XII
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: NM_177963
• Gene symbol: Synaptotagmin-12
• Gene ID (NCBI): 91683
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYT12 belongs to the synaptotagmin family. It may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. The antibody is specific to STY12.

Publikationen

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins.; Authors - Yingchao Li
mouse	WB,IF	Nat Neurosci; Adaptor protein AP-3 produces synaptic vesicles that release at high frequency by recruiting phospholipid flippase ATP8A1; Authors - Hongfei Xu
human	WB	BMC Cancer; Integration of bioinformatics and cellular experiments unveils the role of SYT12 in gastric cancer; Authors - Xingdong Niu