Sam50 Polyklonaler Antikörper

Sam50 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 28679-1-AP

Synonyme

SAMM50, TOB55, SAMM 50, OMP85, CGI-51

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  WB analysis using 28679-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 28679-1-AP (SAMM50 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28679-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (28679-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HeLa using 28679-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28679-1-AP (Sam50 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

28679-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Sam50 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Sam50 fusion protein Ag29152
• Vollständiger Name: sorting and assembly machinery component 50 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 469 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC007830
• Gene symbol: Sam50
• Gene ID (NCBI): 25813
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

Publikationen

Species	Application	Title
human,mouse	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
mouse	IF	Front Cardiovasc Med; Samm50 Promotes Hypertrophy by Regulating Pink1-Dependent Mitophagy Signaling in Neonatal Cardiomyocytes; Authors - Ran Xu
mouse	WB	Int J Biol Sci; Loss of MTX2 causes mitochondrial dysfunction, podocyte injury, nephrotic proteinuria and glomerulopathy in mice and patients; Authors - Ting Li
mouse,rat	WB,IF,IP	Cell Signal; Mitochondrial outer membrane protein Samm50 protects against hypoxia-induced cardiac injury by interacting with Shmt2; Authors - Yufei Zhou
human	WB	Cancer Res Commun; N-Myristoyltransferase Inhibition causes Mitochondrial Iron Overload and Parthanatos in TIM17A-dependent Aggressive Lung Carcinoma; Authors - Sofia Geroyska
human	WB	J Cell Sci; Regulation of mitochondrial cristae organization by Myo19, Miro1/2 and Metaxin 3; Authors - Samruddhi S Shembekar