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Siglec-15 Polyklonaler Antikörper

Siglec-15 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28594-1-AP

Synonyme

CD33 antigen like 3, CD33L3, HsT1361, SIGLEC 15, SIGLEC15

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of A549 cells using Siglec-15 Polyclonal antibody (28594-1-AP)

    WB analysis of A549 using 28594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28594-1-AP (Siglec-15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, NCI-H1299-Zellen, PC-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

28594-1-AP bindet in WB, ELISA Siglec-15 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Siglec-15 fusion protein Ag29829
Vollständiger Name sialic acid binding Ig-like lectin 15
Berechnetes Molekulargewicht 36 kDa
Beobachtetes Molekulargewicht38-40 kDa
GenBank-ZugangsnummerNM_213602
Gene symbol SIGLEC15
Gene ID (NCBI) 284266
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sialic acid binding Ig-like lectin 15 (Siglec-15) is a member of the Siglec family of glycan-recognition proteins. Siglec-15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain, and a short cytoplasmic tail (PMID: 17483134). It is mainly expressed on a subset of myeloid lineage cells and can be upregulated in many cancers (PMID: 32939323). Siglec-15 has been reported as a immune suppressor and a potential target for normalization cancer immunotherapy (PMID: 30833750). Siglec-15 has a calculated molecular weight of 36 kDa. It has been detected at 38-40 kDa due to glycosylation (PMID: 32921411).

Protokolle

Produktspezifische Protokolle
WB protocol for Siglec-15 antibody 28594-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Rebecca (Verified Customer) (07-21-2022)

Positive staining identified at 1:500 dilution in patient FFPE tissue. Slight background.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Prostate cancer FFPE tissue
Siglec-15 Antibody Immunofluorescence validation (1:500 dilution) in Prostate cancer FFPE tissue (Cat no:28594-1-AP)