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Stomatin Monoklonaler Antikörper

Stomatin Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A4D6

Kat-Nr. : CL488-67894

Synonyme

BND7, EPB7, EPB72, Protein 7.2b, STOM, stomatin

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated Stomatin Monoclonal (CL488-67894)

    FC experiment of A549 using CL488-67894

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Stomatin (CL488-67894, Clone:1A4D6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCA549-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67894 bindet in FC (Intra) Stomatin und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Stomatin fusion protein Ag2676
Vollständiger Name stomatin
Berechnetes Molekulargewicht 288 aa, 32 kDa
Beobachtetes Molekulargewicht32 kDa
GenBank-ZugangsnummerBC010703
Gene symbol STOM
Gene ID (NCBI) 2040
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 488 Stomatin antibody CL488-67894Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen